2 with 1000 cells. Microplate Spectrophotometer. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. 20 attomol ATP per well. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. SpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. 313 reference gain check fail. 20 attomol ATP per well. Recertification service. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Select Update Driver. 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. 384-Well SBS Microplate, clear, square well, flat bottom, sterile, with cover, working volume 90 µl, packaged in sleeves of 10. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. For more sample throughput, the system canPlate Background OD, which was used for subsequent tests, was measured as follows. Contact Us Phone: +1-800-635-5577 Web: Email: [email protected] nM Z Factor at EC. ability to use as little as 2˜µL samples with 24- or 64-well plates. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. Source plate capacity; 1 x plates. Algorithms are optimized for accurate cell detection and. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. 5 mP 1536–2. Important things to consider when purchasing a multimode microplate reader. These lab workhorses are used in drug discovery, research, bioassay validation, and. Versa Max. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. and the A1 corner of the plate adapter (Figure 1-2). The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. Temperature control. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. It. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Plate chamber design of the SpectraMax category 1 microplate readers. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. . Fluorometers also vary in the size of samples they accept, with some using very small sample sizes to conserve precious sample materials (down to 0. FlexIII. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 1. 96-384. 5577. Molecular Devices SpectraMax Plus 384 Absorbance Reader Details. …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Selects either 96-well or 384-well plate for a stand-alone read. 2. the use of a reader calibrated with primary NIST and NMI standards. Recertification service. Ideal for miniaturized 384 well fluorescent. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). Validated by Molecular Devices for the FLIPR reader. 003 fmol 0. Application Area: Spectrophotometer for molecular biology. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. For Research Use Only. Danaher Inc 384 well plate 384 Well Plate, supplied by Danaher Inc, used in various techniques. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. Dispense 10 µL of each ATP/ADP combination across an entire row of a 384-well plate. #ABS Plus). A 96- and 384-well automated washer. x. Add 10 µL of ADP Detection Mix to those rows. 76 L x 85. 6-384 (96- and 384-well with pipetting) 96-well microplates. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Temperature control: 4°C above ambient to 45°C. White plates reflect the light, enabling even low levels of Luminescence to be detected. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. x. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modes<2 fg/well for firefly luciferase in 96- and 384- well top read. Supplier: Molecular Devices. Plate readers, also known as. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 306 plate air cal. , April 14, 2022 – Molecular Devices, LLC. For more sample throughput, the system canabsorbance plate readers. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. VIS absorbance detection. FlexStation® 3 Multi-Mode MicroplateAlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. By measuring optical pathlength, it. Microplate format. 4 decades . U-bottom: 30-40 µl. Plate reader instructions Computer. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). Related products. x. RUO. Alpha 1536 HTS 96, 384, and . Incubate the plates at room temperature for 20 min before reading plate luminescence using a Paradigm plate reader (Molecular Devices) (see Note 14). What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the. Turn on Plate Reader and wait for it to complete its start-up routine. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Programmable 10% to 100%: Sample Cell Options: 96-, 384- or 1536-well microwell plates. 96/384 - For the SpectraMax 340PC384, press to select to read a 96-well plate or a 384- well plate. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. (1536-well plate) 2 µL (quartz cuvette), 4 µL (disposable cuvette). Turn the reader on and secure the USB cable. In addition, SoftMax Pro SoftwareBelow is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. With optimized reagents, option to operate the readers. It also integrates with the. There is no product in the shopping cart, buy it!. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. Published Dec 16, 2020 | Updated Dec 16, 2020. They perform rapid throughput cell-based assays while. • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. 9. x. Packed 25 plates per sleeve, 2 sleeves per case. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. flat-bottom: 50-60 µl. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Includes PC and software. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Microplates, cuvettes (via adapter) Detectors. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. This kit is designed for customers who. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. Some instruments can handle only 96-well plates and lower, whereas others can read up to the ultrahigh throughput densities of 1,536-well plates. Backed by the. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. Microplate Readers Molecular Devices Spectramax M3 Multi Mode Microplate Reader. 384-well SBS microplates are designed with corners cut at a1 and h1 positions to conform to SBS nomenclature. Cell viability는 세포막 Integrity 또는. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. Figure 1. Page 1 of 8 . 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. Microplate readers can be used to quantify this turbidity to monitor events such as cell growth or to perform solubility screens. Dynamic range > 6 decades . Other technologies such as imaging. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Extended linear dynamic range. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. The FLEXstation II 384 (Molecular Devices, Inc. #0200-7011) Instrument setupRound well design with a solid black flat bottom2. Molecular Devices: n/a:. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. This unlocks the transport slide. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. Experience the identifiably different. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. To prevent evaporation, the microplate was covered with a qPCR seal. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. M5. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 384- or 96-well read plate containing cells loaded. Four wash/rinse bottles are included and configurable for both 96- and 384. 1536-well plates < 100 amol (384-well) 1 HTRF • Cisbio HTRF detection with optimized Xenon light source and . Round well design with a solid black flat bottom2. Option 1: Use a plate. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. 96/384 - For the SpectraMax 340PC384, press to select to read a 96-well plate or a 384- well plate. 6-384 (96- and 384-well with pipetting) 96-well microplates. Made from polypropylene with conical well profiles to maximize recovery and allow working with sample volumes as low as 5 µL. BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. Click SoftMax Pro Software User Guide. 48 W x 12. flat meniscus), but is approx 15 mL. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Results Microplate readers read biological, chemical or physical events that occur on a microtiter plate. Fluorescence Microplate Reader Comparison Chart. 2 OD at 200 nm; this decreased to 0. Expanded wavelength coverageIn the software select Tools > Configuration. B. The analyzed results are. Published Dec 16, 2020 | Updated Nov 07, 2022. This application is highlighted by the example of a fully automated kinetic solubility screen in 384-well plate format. 3860 N First Street. the Gemini XPS reader. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. +1 800. 5 pM 2. x. Microplate format. Gemini EM Reader scans. No accessories are needed and SoftMax® Pro Software. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 303 unable to cal dark current. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. 1. and the A1 corner of the plate adapter (Figure 1-2). The FLIPR Fluorometric Imaging Plate Reader system addresses the industry's requirements for higher throughput, minimized sample volume, and cost reduction. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Values are averages from 5 clean plates, each filled with 200 µL water/well. Instrument settings are listed in Table 1. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. x. 384-well plates • Automated rinse reduces clogging • Quiet and efficient. Learn more about SpectraMax Plus 384 Microplate Reader >>Rating: 5. 1 pM 1536-well: 0. Three low volume 24-well bottom slides. This can increase throughput and efficiency, thus saving time and space in the lab. 1. Figure 1. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. #R8190 (10) vials of component A*Instruments with a 96- or 384-well format will accommodate the most commonly used plates. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-wellThe FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. The VICTOR Nivo. Category 2 and 3 microplate readers use gradient heating as well. Step 1: Open the SoftMax Pro IMAP FP protocol. Therefore, it is recommended that the pipettor height to be set to correspond to the fluid volume in the cell plate prior to compound addition. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. ) 45 (W) x 70 (H) x 27 (D) Dimensions (cm) 114 (W) x 178. Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)… Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Align the two slots on the bottle holder with the two pegs on the rail. Microplate Readers. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Streamline your workflow with comparative confluence assays to identify and verify gene edits. 340 PC384. 7. Artel MVS Bundle DM Std/800TSNB Reader Details. Temperature control: 4°C above ambient to 45°C. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. Both formulations utilize Molecular Devices proprietary quench technology to enhance signal windows and yield acceptable Z-scores to screen a variety of targets, including TRP, ligand-, cyclic nucleotide- and voltage-gated channels. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the. 0 out of 5 $ 8,495. *For Corning UV plates, mean values at 260 nm ranged from 0. We would like to show you a description here but the site won’t allow us. 3% in white 96- and 384-well microplates < 3 x 10-5 (white plates) < 3 x. 2. Features. This chapter describes assays where data are recorded using a plate-reader; it does not cover assay methods designed for flow cytometry or high content imaging. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. Wash heads are made of Polyethylene Terephthlate (PET) with stainless steel probes to prevent corrosion. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. Biotek Synergy 2 Plate Reader Multi-Mode. Outer wells aligned for proper viewing with FLIPR Ideal. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 4 decades . 384-Well Microarray/High Sample Recovery Plate. 2. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. 384-well TC plate, Poly-d-Lysine, Flat Clear. 1 OD at 230 nm. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. ©2017 Molecular Devices, LLC . Shaking: Linear and Orbital. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 5 ml microcentrifuge tubes 35 mm Petri dishes 12 mm × 50 mm test tubes (optional). To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andSupplier: Molecular Devices. For SpectraMax L or LMax II, LMax II 384 - no adapter used. 384-Deep Square Well Plates. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Washers configured for both 96- and 384-well plates. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. Wash heads are made of Polyethylene Terephthlate (PET) with stainless steel probes to prevent corrosion. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. x. x. were pipetted into a 96-well black-walled μclear plate. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Comprehensive solution. The plate was mixed gently for 2 minutes on a plate shaker and then allowed to incubate at room temperature for 10 minutes to stabilize luminescence. That explains the lower values on the LmaxII384 plate reader. Pro, Dual Read mode was selected. 2. A thermal cycling reaction was immediately initiated. TNF standard curve. 5in (46. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Fluid dispensing speed. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. SpectraMax M2 and M2e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. 1: The SPECTRAmax 340PC 384 System The on-board microprocessor calculates and reports the absorbance (or % Transmittance) for each well of a microplate. Another consideration, says Amouretti, is plate format. B. Supplier: Molecular Devices. For nearly 30 years, Molecular Devices has provided scientists with . " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. 384 well plates 6–384 wells 6–384 wells 6–384 wells 6–1,536 wells (fluorometry, TRF, luminometry, AlphaScreen assays). Fluorescent Imaging Plate Reader (FLIPRTM ) technology to measure Ca2+ flux in cells was an important development in the early 1990’s and has played a significant role in HTS and lead optimization applications. Polypropylene deep well plates: Elevated well walls for easier sealing Round. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications. San Jose, Calif. Suitable for use in BMG lab tech, Molecular Devices, Promega Glomax and other plate readers; Suitable for adhesive and heat sealing: our Moisture Barrier Seal 384. Programmable 10% to 100%: Sample Cell Options: 96-, 384- or 1536-well microwell plates. (96- and 384-well with pipetting) 6-384. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. Figure 1. For more sample throughput, the system canPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The FlexStation 3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Documentation:Molecular Devices microplate readers. Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)…Elisa Plate Readers and Microplate Readers at Biocompare. Automated live cell imaging – cell counting and confluence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The FilterMax F3 can read 96-well and 384-well plates. Instrument pricing. Two high-energy xenon flash lamps: Reading capabilities. The fluid surface in a 384-well is a deeply curved meniscus. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). After 30 minutes the plate was read in Analyst GT with varying z heights. ©2010 Molecular Devices, Inc. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. " A versatile UV/VIS spectrophotometer for both 96- and 384-well. Fluorescent reading data were analyzed by non-linear fitting (GraphPad Prism, GraphPad Software Inc. 5 pM 384-well (50 µL) 6 pM 10 pM 1536-well (8 µL) 19 pM 25 pM 0200-7015 (LUM 384. 96- and 384-well microplates. 96/384-well plate formats. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. #PLUS 384) Methods. Get Quote . The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. com. Dimensions: 18. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Integrated stacker option. Molecular Devices has provided scientists with tools to expand the boundaries of their research. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. REF (SpectraMax Plus 384 only) A reading of buffer, water, or air taken in the cuvette that is used as I 0 to calculate Absorbance or % Transmittance. Products : Ionworks HT, Ionworks Quattro.